Custom Services › Custom Rabbit Antibody Production
Abcore is a Southern California antibody production company focused on making high quality antibodies. We emphasize animal health, and our rabbits are bred and put into production on the same farm. This relieves the stress associated with transportation and adjustment to a new environment which happens in a traditional production environment. Abcore also has on site veterinarians which monitor rabbit health and comfort. Also, our animal facility is USDA inspected and approved (93-R-0574) and NIH/OLAW certified (# A4713-01).
Abcore offers standard 60 day rabbit antibody production packages which consist of 4 immunizations, and 3 production bleeds (please see example project timeline below). However, we can adjust our protocol if you have specific needs. We typically use 2 New Zealand White Rabbits for each project, and prices start at $725.
Abcore provides free antigen design to assist if a peptide immunogen is required. We assist in selecting the best possible peptide sequence for eliciting the desired antibody response. We use the latest in software with the proper algorithms to assess antigenicity, hydrophilicity, flexibility, secondary structure and aggregation potential. Please take advantage of this free assistance if you require a peptide for your custom rabbit antibody production project.
Rabbit
- 60 day production packages
- 4 immunizations
- 3 production bleeds
- 2 New Zealand White Rabbits for each project
Packages start at $720 USD
Pricing
Features |
Package 1 Customer Antigen to Antiserum |
Package 2 Customer Antigen to Protein A/G Purified Antibody |
Package 3 Customer Antigen to Affinity Purified Antibody |
Package 4 Peptide Synthesis to Antiserum |
Package 5 Peptide Synthesis to Protein A/G Purified Antibody |
Package 6 Peptide Synthesis to Affinity Purified Antibody |
Package 7 Affinity Purified Phospho-specific Antibody |
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service includes |
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Prebleed |
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Immunization of 2 rabbits |
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ELISA titre |
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Customer provided antigen |
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Protein A/G purification |
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Affinity purification |
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Antigen design |
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Peptide antigen synthesis |
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Time (weeks) |
8-10 | 10-12 | 10-13 | 12-13 | 13-15 | 12-13 | 13-16 |
what you recieve |
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Full project report |
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ELISA data |
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Preimmune serum (5mL) |
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Rabbit antiserum (125mL) |
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Protein A/G purified antibody (25mL) |
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Affinity purified antibody (25mL) |
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Affinity resin (10mL) |
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Peptide (2-3mg) |
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what you recieve |
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Price |
$725 |
$975 |
$1,225 |
$1,125 |
$1,375 |
$1,695 |
$3,250 |
Additional items if Project is Extended Past 56 Days
Per diem board and care
$2.00/Day/Animal (includes egg collection)
Additional antigen needed
$10.00/ml
Schedule
Week | Action |
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Pre-immunization bleed only upon request | |
0 |
Immunize rabbits with 0.25 mg of antigen mixed with Complete Freund's Adjuvant |
2 |
Immunize rabbits with 0.2 mg of antigen mixed with Incomplete Freund's Adjuvant |
4 |
Immunize rabbits with 0.2 mg of antigen mixed with Incomplete Freund's Adjuvant |
5 |
Production bleed yielding approximately 20-25 ml rabbit serum |
6 |
Immunize rabbits with 0.2 mg of antigen mixed with Incomplete Freund's Adjuvant |
7 |
Production bleed yielding approximately 20-25 ml rabbit serum |
8 |
Production bleed yielding approximately 20-25 ml rabbit serum |
ELISA, western blot or other testing as requested by client | |
Shipment or Purification of Serum |