Abcore has built an entire platform for single domain antibody and VHH discovery. Our process is based on phage display and using either naïve phage libraries or immunized phage libraries for single domain antibody discovery.
Immunized Antibody Phage Libraries:
- Custom Llama Single Domain Antibody Discovery
- Custom Alpaca Single Domain Antibody Discovery
Naïve Antibody Phage Libraries:
- Naïve Llama Single Domain Antibody Phage Library
- Naïve Alpaca Single Domain Antibody Phage Library
- Naïve Camel Single Domain Antibody Phage Library
- Naïve Shark IgNAR Phage Library
- Naïve Rabbit ScFv Phage Library
The single domain antibody discovery process can be broken into phases, based on the goals of the VHH discovery project:
- Immunization
- PBMC Isolation
- cDNA generation
- Phage Library Construction
- Phage Library Panning
- Screening for Positive Clones
- Production of Purified Single Domain VHH Antibodies
- Other CRO Services (Humanization, Functional Assays, Expression Vector changes, etc.)